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Expression stability of common housekeeping genes is differently affected by bowel inflammation and cancer: implications for finding suitable normalizers for inflammatory bowel disease studies.
Inflamm Bowel Dis. 2014 Jul;20(7):1147-56. doi: 10.1097/MIB.0000000000000067.
Inflamm Bowel Dis. 2014.
PMID: 24859296
The pairs RPS23/RPLP0, PGK1/MRPL19, or PPIA/RPLP0 were optimal reference by CV%, NormFinder, and geNorm, respectively. Colon inflammation affected HKG more pronouncedly than cancer with ACTB significantly down- and B2M upregulated. ...
The pairs RPS23/RPLP0, PGK1/MRPL19, or PPIA/RPLP0 were optimal reference by CV%, NormFinder, and geNorm, respectively. Colon infla …
Selection of reference genes for gene expression studies in human bladder cancer using SYBR-Green quantitative polymerase chain reaction.
Zhang C, Wang YQ, Jin G, Wu S, Cui J, Wang RF.
Zhang C, et al.
Oncol Lett. 2017 Nov;14(5):6001-6011. doi: 10.3892/ol.2017.7002. Epub 2017 Sep 19.
Oncol Lett. 2017.
PMID: 29113238
Free PMC article.
Subsequently, the stability of the selected reference genes was analyzed using three different methods: geNorm, NormFinder and BestKeeper. The rarely used ribosomal protein S23 (RPS23) was the most stable single reference gene, with RPS23, tumor protein, translation …
Subsequently, the stability of the selected reference genes was analyzed using three different methods: geNorm, NormFinder and BestKeeper. T …
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